Fig. 2.

LncMAPK6 is overexpressed in liver tumor and TICs. a MAPK6 locus was shown. There is a divergent lncRNA (ENST00000561318, termed as lncMAPK6 in this research) in near from MAPK6 locus according to UCSC Genome Browser. b LncMAPK6 expression levels were detected using Northern blot. 18S rRNA was a loading control. c 19 peri-tumor, 7 eHCC and 12 aHCC were used for In situ hybridization (ISH). Representative photos and indicated ratios were shown. d LncMAPK6 expression levels in CD133⁺ liver TICs and CD133⁻ non-TICs were detected by realtime PCR, and non-TIC expression was used for data normalization. e Spheres and non-spheres were selected, and lncMAPK6 expression was examined with realtime PCR. f, g FISH assay confirmed the high expression of lncMAPK6 in liver TICs (f) and oncospheres (g). Zic2 and CD133 were markers of liver TICs. Scale bars, C, 50 μm; F, 20 μm