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. 2019 Dec 19;17:109. doi: 10.1186/s12958-019-0554-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Bland-Altman plot. Bland-Altman plot shows the differential bias between the percentage of cells detected as capacitated by individual methods after 240 min of incubation and the percentage of cells detected as acrosome-reacted by PSA after ZP-induced AR. The zero baseline represents the percentage of cells detected as acrosome-reacted by PSA, individual color lines shows the biases for CTC, ACR.2, FITC-phall and pY assays. Circles represents individual data points (N = 20 for each method), dotted lines represent 95% LA (Limits of agreement)