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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Aug 23;1865(12):165538. doi: 10.1016/j.bbadis.2019.165538

Figure 4. Generation of MUT-null and PCCA-null HEK293 cells.

Figure 4.

(A) Immunoblot shows absent expression of PCCA in PCCAE5Δ13 cells (n=3, serial passages) compared to WT cells (n=3, serial passages). β-actin was used as a loading control. (B) Immunoblot shows absent expression of MUT in MUTE5Δ22 cells (n=3, serial passages) compared to WT cells (n=3, serial passages). β-actin was used as a loading control. (C) Sanger sequencing trace of PCCAE5Δ13 highlights a 13 bp deletion in exon 12 of PCCA. (D) Sanger sequencing trace of MUTE5Δ22 cells highlights a 22 bp insertion within exon 5 of MUT.