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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Aug 23;1865(12):165538. doi: 10.1016/j.bbadis.2019.165538

Fig. 6.

Fig. 6.

mRNA expression in PCCAE12Δ13 and MUTE5Δ22 cells at 4 and 8 days of growth. (A) At day 4 (log phase of growth), MUTE5Δ22 had increased expression of serine transporter gene SLCJA5 (1.6–fold) and SLC3A2 (1.7–fold) compared to WT, though this did not reach statistical significance. Additionally, PCCAE12Δ13 had significantly decreased expression of both serine transporters gene SLCJA5 andSLC3A2 (0.3 fold and 0.5–fold, respectively) compared to WT. (B) At day 8 (cells at confluence), PCCAE12Δ13 and MUTE5Δ22 had increased expression of ASNS (1.4–fold and 1.7–fold, respectively) and PSAT1 (2.0–fold and 3.1–fold, respectively) compared to WT. Additionally, PCCAE12Δ13 had significantly decreased expression of SLC7A5 (0.1–fold) and SLC3A2 (0.2–fold) compared to WT. (n=3, 3 individual 6–well culture wells per genotype per time point) (n=3, 3 individual 6–well culture wells analyzed per genotype per time point). * indicates a statisticially significant change (p < 0.05) compared to WT.