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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Aug 23;1865(12):165538. doi: 10.1016/j.bbadis.2019.165538

Figure 8. 13C-labeled TCA intermediates MUTE5Δ22and PCCAE12Δ13 cells.

Figure 8.

(A) 13C labeled pyruvate is not significantly different in and MUTE5Δ22 (n=5, separate culture dishes) and PCCAE12Δ13 cells (n=5, separate culture dishes) compared to WT (n=5, separate culture dishes) (B) Citrate is significantly lower in MUTE5Δ22 compared to WT (0.7-fold, p=0.045). (C) Aconitate is significantly lower in MUTE5Δ22 compared to WT (0.7-fold, p=0.035). (D) Isocitrate is not significantly different between cell types. (E) Alpha-ketoglutarate is significantly lower in MUTE5Δ22 compared to WT (0.6-fold, p=0.025). (F) Fumarate is significantly lower in MUTE5Δ22 and PCCAE12Δ13 compared to WT (0.6-fold (p=0.002) and 0.5 fold (p=1.9E−04) respectively). (G) Malate is significantly lower in MUTE5Δ22 and PCCAE12Δ13 compared to wT (0.6-fold (p=7.8E−04) and 0.6 fold (p=3.6E−05) respectively). Values (y-axis) are represented as average Ms peak heights +/− standard deviation for each genotype. Raw values were corrected for protein content.13C labeled succinate, succinyl CoA, oxaloacetate and acetyl CoA were not measured in this assay due to technical limitations.