Table I.
Association between DUBs and clinicopathological variables and survival in patients with gastric cancer.
| Age, years | Sex | Tumor size, cm | Lauren classification | TNM stage | 5-year survival | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DUB | Expression | Cases | <60 | ≥60 | M | F | <5 | ≥5 | Intestinal | Diffuse | I and II | III and IV | Patients, n | Rate (%) |
| BAP1 (30) | Positive | 211 | 124 | 87 | 145 | 66 | 42 (<3) | 169 (≥3) | 51 | 117 | 91 | 74 | 136 | 65 |
| Negative | 263 | 144 | 119 | 180 | 83 | 20 (<3) | 243 (≥3) | 43 | 174 | 120 | 189 | 100 | 38 | |
| P-value | 0.381 | 0.948 | 0.001 | 0.042 (Mix=43/46) | 0.01 | 0.001 | ||||||||
| UCHL1 (29) | Positive | 87 | 37 | 50 | 67 | 20 | 23 (<3) | 64 (≥3) | NF | NF | 25 | 62 | 26 | 30 |
| Negative | 109 | 59 | 50 | 80 | 29 | 43 (<3) | 66 (≥3) | NF | NF | 53 | 56 | 66 | 61 | |
| P-value | 0.107 | 0.561 | 0.038 | NF | 0.004 | 0.001 | ||||||||
| UCHL5 (28) | Positive | 379 | 181 (<66) | 198 (≥66) | 188 | 191 | 139 | 240 | 181 | 198 | 150 | 229 | 258 | 65 |
| Negative | 111 | 51 (<66) | 60 (≥66) | 54 | 57 | 50 | 61 | 34 | 77 | 40 | 71 | 42 | 38 | |
| P-value | 0.747 | 0.914 | 0.173 | 0.004 | 0.001 | 0.108 | ||||||||
| USP10 (45) | Positive | 198 | 95 (≤57) | 103 (>57) | 137 | 61 | 83 (<4) | 115 (≥4) | 111 | 87 | 108 | 90 | 106 | 64 |
| Negative | 167 | 85 (≤57) | 82 (>57) | 118 | 49 | 60 (<4) | 107 (≥4) | 72 | 95 | 69 | 98 | 79 | 40 | |
| P-value | 0.578 | 0.761 | 0.182 | 0.060 | 0.004 | 0.003 | ||||||||
| USP14 (44) | Positive | 62 | 36 | 26 | 49 | 13 | NF | NF | NF | NF | 17 | 45 | NF | NF |
| Negative | 51 | 32 | 19 | 44 | 7 | NF | NF | NF | NF | 21 | 30 | NF | NF | |
| P-value | 0.613 | 0.316 | NF | NF | 0.124 | NF | ||||||||
| USP20 (57) | Positive | 52 | 21 | 31 | 37 | 15 | 39 | 13 | NF | NF | 30 | 22 | 23 | 45 |
| Negative | 37 | 18 | 19 | 24 | 13 | 11 | 26 | NF | NF | 13 | 24 | 8 | 20 | |
| P-value | 0.439 | 0.529 | 0.001 | NF | 0.036 | 0.003 | ||||||||
| USP22 (58) | Positive | 125 | 55 | 70 | 88 | 37 | 56 | 69 | NF | NF | 23 | 102 | 23 | 18 |
| Negative | 94 | 42 | 52 | 74 | 20 | 39 | 45 | NF | NF | 59 | 35 | 45 | 48 | |
| P-value | 0.920 | 0.165 | 0.283 | NF | 0.004 | 0.001 | ||||||||
| USP3 (71) | Positive | 67 | NF | NF | 42 | 25 | NF | NF | 37 | 30 | 16 | 51 | 13 | 20 |
| Negative | 80 | NF | NF | 25 | 27 | NF | NF | 61 | 19 | 50 | 30 | 48 | 60 | |
| P-value | NF | 0.653 | NF | 0.007 | 0.001 | 0.001 | ||||||||
| USP33 (72) | Positive | 58 | 24 (≤55) | 34 (>55) | 32 | 26 | 38 | 20 | NF | NF | 34 | 24 | 40 | 68 |
| Negative | 63 | 23 (≤55) | 40 (>55) | 19 | 32 | 32 | 31 | NF | NF | 12 | 51 | 26 | 42 | |
| P-value | 0.583 | 0.095 | 0.182 | NF | 0.001 | 0.001 | ||||||||
| USP39 (74) | Positive | 26 | NF | NF | NF | NF | NF | NF | NF | NF | NF | NF | 6 | 24 |
| Negative | 27 | NF | NF | NF | NF | NF | NF | NF | NF | NF | NF | 18 | 62 | |
| P-value | NF | NF | NF | NF | NF | 0.019 | ||||||||
| USP42 (85) | Positive | 45 | 15 (<55) | 30 (≥55) | 36 | 9 | 25 | 20 | NF | NF | 49 | 41 | NF | NF |
| Negative | 45 | 11 (<55) | 34 (≥55) | 31 | 14 | 14 | 31 | NF | NF | 18 | 27 | NF | NF | |
| P-value | 0.486 | 0.334 | 0.033 | NF | 0.006 | NF | ||||||||
| USP44 (86) | Positive | 90 | NF | NF | 60 | 30 | NF | NF | NF | NF | 32 | 58 | 33 | 36.8 |
| Negative | 117 | NF | NF | 78 | 39 | NF | NF | NF | NF | 51 | 66 | 59 | 50.5 | |
| P-value | NF | 1 | NF | NF | 0.440 | 0.033 | ||||||||
| USP9X (97) | Positive | 43 | 31 | 12 | 34 | 9 | 29 | 14 | NF | NF | 11 | 32 | 4 | 10 |
| Negative | 25 | 15 | 10 | 18 | 7 | 12 | 13 | NF | NF | 16 | 9 | 8 | 32 | |
| P-value | 0.304 | 0.508 | 0.114 | NF | 0.006 | 0.003 | ||||||||
| OTUB1 (105) | Positive | 78 | 33 | 45 | 56 | 22 | 35 | 43 | NF | NF | 10 | 68 | 20 | 25 |
| Negative | 78 | 40 | 38 | 64 | 14 | 56 | 22 | NF | NF | 22 | 56 | 33 | 42 | |
| P-value | 0.261 | 0.128 | 0.001 | NF | 0.017 | 0.027 | ||||||||
| EIF3F (133) | Positive | 66 | 33 | 33 | 49 | 17 | 34 | 32 | NF | NF | 53 | 13 | 56 | 85 |
| Negative | 129 | 51 | 78 | 94 | 36 | 80 | 49 | NF | NF | 46 | 90 | 90 | 70 | |
| P-value | 0.170 | 0.870 | 0.160 | NF | 0.020 | 0.040 | ||||||||
The expression of DUBs in GC and normal tissues was not significantly associated with sex or age, but was associated with tumor size, tumor stage, grading and 5-year-survival. Positive expression refers to the positive expression of a certain protein in gastric cancer. All expression was detected by immunohistochemistry. BAP1, BRCA1 associated protein-1; DUBs, deubiquitinating enzymes; EIF3F, eukaryotic translation initiation factor 3 subunit F; NF, not found; OTUB1, otubain 1; TNM, tumor-node-metastasis; UCH, ubiquitin C-terminal hydrolase; USP, ubiquitin-specific protease. *P<0.05.