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. 2019 Dec 19;50:110. doi: 10.1186/s13567-019-0728-x

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© The Author(s) 2019

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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EGTA treatment has no effect on the post-internalization stage of rotavirus particle in enterocytes. Cells were inoculated with rotavirus at 37 °C for 1 h. After 3 washings, cells were treated with EGTA or PBS for 30 min. Then, the cells were further incubated for 9 h at 37 °C. A Representative confocal image of infection in enterocytes. Scale bar: 50 µm. The percentage of infected cells (B) and virus titer (C) were determined. Data are expressed as the mean ± SD of the results of three separate experiment.