Table 3.
Effects of L. acida on spermatozoa abnormalities in colibri®-exposed rats
| Treatments | Doses | Head abnormality | Tailless SPZ | Tail abnormality | Cytoplasmic droplet |
|---|---|---|---|---|---|
| Untreated | 10 ml/kg | 7.81 ± 0.25 | 0.60 ± 0.43 | 15.80 ± 0.92 | 0.34 ± 0.21 |
| 14 Days | |||||
| Colibri® + DW | 10 ml/kg | 32.00 ± 3.16### | 5.99 ± 1.65 | 52.90 ± 1.16### | 5.68 ± 1.81 |
| Colibri® + CC | 2 mg/kg | 13.14 ± 1.04*** | 2.27 ± 0.48 | 34.52 ± 1.53*** | 2.68 ± 0.98 |
| Colibri® + Vit E | 75 mg/kg | 27.84 ± 4.27 | 3.27 ± 0.78 | 23.19 ± 1.27*** | 4.36 ± 2.27 |
| Colibri® + AE | 170 mg/kg | 11.30 ± 2.05*** | 3.54 ± 1.05 | 28.84 ± 2.51*** | 2.35 ± 0.64 |
| Colibri® + AE | 340 mg/kg | 8.46 ± 1.01*** | 1.45 ± 0.46* | 27.17 ± 1.69*** | 1.81 ± 0.61 |
| Colibri® + ME | 170 mg/kg | 4.58 ± 1.28*** | 1.74 ± 0.31* | 23.72 ± 1.16*** | 0.66 ± 0.31 |
| Colibri® + ME | 340 mg/kg | 8.82 ± 1.50*** | 1.17 ± 0.33** | 24.52 ± 1.56*** | 0.67 ± 0.17 |
| 28 Days | |||||
| Colibri® + DW | 10 ml/kg | 23.94 ± 2.54### | 11.51 ± 6.40 | 40.55 ± 9.38## | 3.96 ± 1.17### |
| Colibri® + CC | 2 mg/kg | 12.27 ± 2.53* | 4.48 ± 1.97 | 20.87 ± 2.01*** | 0.69 ± 0.31** |
| Colibri® + Vit E | 75 mg/kg | 26.90 ± 4.13 | 2.23 ± 1.21 | 16.43 ± 1.45*** | 2.16 ± 0.38 |
| Colibri® + AE | 170 mg/kg | 7.76 ± 1.30*** | 1.72 ± 0.77 | 14.71 ± 2.00*** | 0.67 ± 0.31** |
| Colibri® + AE | 340 mg/kg | 4.78 ± 0.50*** | 0.99 ± 0.30 | 17.08 ± 2.27*** | 1.30 ± 0.42* |
| Colibri® + ME | 170 mg/kg | 10.38 ± 0.83** | 2.26 ± 1.35 | 15.12 ± 1.57*** | 0.34 ± 0.34*** |
| Colibri® + ME | 340 mg/kg | 5.94 ± 1.41*** | 3.38 ± 0.68 | 15.21 ± 1.32*** | 0.79 ± 0.44** |
Number of rats per group = 5. All values are expressed as mean ± SEM
SPZ spermatozoa, DW distilled water, CC clomiphene citrate, Vit E vitamin E, AE aqueous extract, ME Methanol extract
##: p ≤ 0.01; ###: p ≤ 0.001 significantly different compared with untreated animals *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001: significantly different compared with colibri® + DW group