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. 2019 Nov 13;19(1):519–526. doi: 10.3892/ol.2019.11090

Figure 4.

Figure 4.

Effects of oxymatrine on the modulation of epithelial-mesenchymal transition proteins in HCT-8/5-FU cells. (A) Western blot analysis of proteins extracted from HCT-8/5-FU cells treated with 2 mg/ml oxymatrine and 20 ng/ml TNF-α, alone or in combination, for 24 h. (B) Quantification of western blot analysis. *P<0.05 vs. control cells. (C) Immunofluorescence staining of HCT-8/5-FU cells treated with 2 mg/ml oxymatrine for 24 h, and then subjected to immunofluorescence staining of E-cadherin (green) and vimentin (red) proteins, and DAPI nuclei staining (blue). Scale bar, 50 µm. Magnification, ×400. p-p65, phosphorylated p65; OMT, oxymatrine; MIX, combined treatment; TNF-α, tumor necrosis factor-α; SNAI2, snail family transcriptional repressor 2.