Figure 3.

CDKN3 knockdown suppresses the proliferation, migration and invasion of ESCC cells. (A) Cell counting kit-8 assay demonstrated that CDKN3 knockdown suppressed the cell proliferative ability. (B and D) Colony formation assay indicated that CDKN3 knockdown inhibited the proliferation of ESCC cells. (C) The results of cell cycle showed that knock down of CDKN3 increased the cells in G0/G1 phase. *P<0.05, **P<0.01 vs. si-NC. CDKN3, cyclin-dependent kinase inhibitor 3; ESCC, esophageal squamous cell carcinoma; si-NC, cells transfected with the negative control small interfering RNA; siCDKN3, cells transfected with small interfering RNA targeting CDKN3; OD, optical density.