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. 2019 Nov 19;19(1):368–378. doi: 10.3892/ol.2019.11116

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Chemotherapeutic drugs induce autophagy in leukaemia cells. (A) Chemotherapeutic drugs damaged the leukaemia cells dose-dependently. Cell viability was measured using a Cell Counting Kit-8. (B) Cell lysates were subjected to western blotting to detect LC3-II/I and p62 expression. β-actin was used as the loading control. Quantified data are presented (p62 or LC3-II/I/β-actin). (a-1) Western blot diagram of RS4:11 cells. (a-2) p62 quantitative data of RS4:11 cells. (a-3) LC3II/I quantitative data of RS4:11 cells. (b-1) Western blot diagram of Jurkat cells. (b-2) p62 quantitative data of Jurkat cells. (b-3) LC3II/I quantitative data of Jurkat cells. (C) LC3 was stained via immunofluorescence and analysed under a confocal microscope to measure the LC3 puncta (LC3, Cy3 staining; nucleus, DAPI staining). (D) Cells were subjected to transmission electron microscopy to observe autophagosome-like structures (indicated by red circles). Data are the mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001, compared with the untreated group. DNR, daunorubicin.