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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: J Am Chem Soc. 2019 Dec 6;141(50):19585–19588. doi: 10.1021/jacs.9b11608

Figure 2.

Figure 2.

Directed evolution of PsEFE for aziridination. (A) Evolutionary lineage. Reactions were performed in triplicate anaerobically with acetate and quantified by analytical HPLC-UV. Full experimental details are given in the Supporting Information. (B) Structural representation of PsEFE with mutated sites highlighted in orange; metal-coordinating residues H189, D191, and H268 are represented in sticks and Mn (the metal with which the protein was crystallized) is represented as a purple sphere (PDB ID: 6CBA).