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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: J Am Chem Soc. 2019 Dec 6;141(50):19585–19588. doi: 10.1021/jacs.9b11608

Table 1.

Aziridination catalyzed by wild-type PsEFE

graphic file with name nihms-1062404-t0002.jpg
Deviation from standard conditions1 Relative activity
None 1.00
No iron 0.08
No αKG 0.52
Acetate2 instead of αKG 6.72
N-oxalylglycine3 instead of αKG 7.75
Acetate instead of αKG, no ascorbic acid 6.01
1

Standard conditions: Reactions were performed in MOPS buffer (20 mM pH 7.0) with 5% ethanol co-solvent, with 20 μM apoenzyme, 1 mM Fe(NH4)2(SO4)2, 1 mM αKG (as disodium salt), 1 mM L-ascorbic acid, and 10 mM 1 and 2.

2

Sodium salt.

3

Free acid.