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. 2019 Dec 2;19(1):745–752. doi: 10.3892/ol.2019.11170

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Copyright: © Jia et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Comparative analysis of cell fixation procedures for determining the optimum conditions for immunodetection of mitotic chromatin-associated HMGB1. The astrocyte HA1800 cell line and three glioma cell lines (U251, U118 and U87) were used for the immunodetection of HMGB1 during mitosis. (A) HMGB1 localization in interphase and mitotic nuclei in PFA-fixed astrocytes and glioma cells. Scale bar=10 µm. (B) HMGB1 localization in interphase and mitotic nuclei in astrocytes and glioma cells fixed with chilled methanol with 5% (v/v) acetic acid. Scale bar=10 µm. DAPI was used to stain the nuclei. HMGB1, high mobility group box 1; PFA, paraformaldehyde; Ip, interphase; Pp, prophase; Mp, metaphase; Ap, anaphase; Tp, telophase.