FIGURE 3.

FGF2-STABs show increased thermal stability, as indicated by their lower EC₅₀ values. (A) The bar plot presents EC₅₀ values of FGF2 variants after thermal treatments, calculated from data presented in Figure 2A. The exclamation mark (!) indicates EC₅₀ values that are only rough estimates from the available data; however, true EC50 values are most likely higher because at the range of concentrations tested, maximum response was not reached. (B) The color maps show results of statistical analysis of EC₅₀ values of FGF2 variants after thermal treatment, calculated from data presented in Figure 2A. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001; ^****P < 0.0001; n.s., not significant (one-way ANOVA, Tukey’s multiple comparisons test). NA, not applicable. (C) Dependence of the melting temperature (T_m) of the FGF2 variants on heparin concentration. The data were fitted to sigmoidal curves. The color maps show results of statistical comparison of whole curves (two-way ANOVA). ^∗∗P < 0.01; ^****P < 0.0001. NA, not applicable.