TABLE 1.

The dependence of EC₅₀ values of FGF2 variants on thermal treatment and heparin.

FGF2 variant	Thermal treatment	EC50 [nM]
		No heparin	With heparin
FGF2-wt	−20°C	0.098	NA
	37°C, 7 days	3.360 (!)	0.258
	37°C, 30 days	4.342 (!)	0.389
	50°C, 24 h	3.450 (!)	0.364
FGF2-STAB1	−20°C	0.006	NA
	37°C, 7 days	0.018	0.019
	37°C, 30 days	0.071	0.040
	50°C, 24 h	0.050	0.022
FGF2-STAB2	−20°C	0.004	NA
	37°C, 7 days	0.008	0.006
	37°C, 30 days	0.013	0.008
	50°C, 24 h	0.008	0.007

The EC₅₀ values were calculated from experiments in BaF3-FGFR2c cells from plots depicted in Figure 1B. The exclamation mark (!) indicates rough estimate values from the available data; however, true EC₅₀ values are most likely higher because at the range of concentrations tested, maximum response was not reached.