Table 1. Three-way ANOVA to assess the effect of genotype and the dispersal status (i.e. difference between dispersers and residents) on three movement metrics: activity (proportion of moving cells), movement speed and linearity.

Genotype and dispersal status were considered as crossed and fixed effects, and replicate as random effect nested in genotype but crossed with dispersal status because data from the two status were paired per replicate (i.e. the start and target tubes of one dispersal system). The column ‘denominator for F-test’ indicates the error term used to test for each effect, according to the ANOVA model; ‘–’ denote the factors that cannot be tested because the error has no degrees of freedom in this model.

Response variable			Activity				Speed: ln (speed)				Linearity: ln (tau)
Factor	Denominator for F-test	DF	SS	MS	F value	p	SS	MS	F value	p	SS	MS	F value	p
Genotype	Replicate (genotype)	43	0.872	0.020	2.88	<0.0001	24.927	0.580	12.40	<0.0001	24.666	0.574	7.50	<0.0001
Dispersal status (disperser vs. resident)	Replicate × dispersal status (genotype)	1	0.186	0.186	42.88	<0.0001	3.193	3.193	149.28	<0.0001	6.718	6.718	93.19	<0.0001
Genotype × dispersal status	Replicate × dispersal status (genotype)	43	0.445	0.010	2.39	0.0003	3.977	0.092	4.32	<0.0001	7.036	0.164	2.27	0.0006
Replicate (genotype)	Error	87	0.612	0.007	–	–	4.067	0.047	–	–	6.653	0.076	–	–
Replicate × dispersal status (genotype)	Error	87	0.377	0.004	–	–	1.862	0.021	–	–	6.272	0.072	–	–
Error	na	0	0	–			0	–			0	–
Total		261	2.490				38.020				51.317