Table 3.
Bacteria tested | Minimum bactericidal concentration (MBC) (µg/ml) | |||||
---|---|---|---|---|---|---|
35ºC/std pH | 35ºC/pH 5.5 | 10ºC/std pH | 10ºC/pH 5.5 | 4ºC/std pH | 4ºC/pH 5.5 | |
Escherichia coli O157:H7 | 10,000 ± 0.00a | 10,000 ± 0.00 | 10,000 ± 0.00a | 8,333.33 ± 2,581.98a | 10,000 ± 0.00a | 8,333.33 ± 2,581.98a |
Escherichia coli O111 | 10,000 ± 0.00a | 10,000 ± 0.00 | 10,000 ± 0.00a | 8,333.33 ± 2,581.98a | 10,000 ± 0.00a | 8,333.33 ± 2,581.98a |
Escherichia coli ATCC 25922 | 7,500 ± 2,594.37a,b | 10,000 ± 0.00 | 7,272.72 ± 2,783.93ª,b | 8,333.33 ± 2,581.98a | 7,272.72 ± 2,783.93ª,b | 8,333.33 ± 2,581.98a |
Enterococcus faecalis | 5,625 ± 2,677.07b | 10,000 ± 0.00 | 5,625 ± 2,677.07b | 8,333.33 ± 2,581.98a | 5,625 ± 2,677.07b | 8,333.33 ± 2,581.98a |
Data are expressed as means ± SD. In each column, different letters mean significant differences (p < .05). 24h of culture at 35ºC, 120 hr of culture at 10ºC, and 240 hr of culture at 4ºC. std pH, standard pH (7.4 for Escherichia coli or 6.7 for Enterococcus faecalis).