Skip to main content
. 2019 Oct 30;3(6):e308. doi: 10.1097/HS9.0000000000000308

Figure 3.

Figure 3

CLL-derived NK cells are hypo-responsive. HC- or CLL-derived NK cells were co-cultured with K562 target cells in indicated E:T ratio's (A), or in a ratio of 1:10 (B-G) for 3 to 4 hours. HC (n = 11) are indicated in white, CLL patients (n = 10) in black. In both groups good responders are depicted as circles, bad responders (<40–60% specific lysis in 1:1 or 2:1 E:T ratio) are depicted as squares. Target cell lysis and NK cell function were analyzed by flow cytometry. (A) Percentage target cell lysis for HC (left), CLL patients (middle) and groups combined (right). (B) Correlation between expression of NKG2D and percentage of target cell lysis at and E:T ratio of 1:1. (C) Expression of NKG2D on HC- or CLL-derived NK cells. (D) Percentage of degranulated (CD107a+) NK cells after co-culture with K562 target cells. (E) Release of granzyme B after co-culture with K562 cells. Granzyme B MFI was normalized to the MFI of NK cells that were not cultured with K562 target cells. (F) Activation of NK cells after co-culture with K562 cells, measured as the frequency of phospho-S6 high NK cells. (G) Percentage of IFNγ producing NK cells after co-culture with K562 cells. R = responder, NR = non-responder. Bars indicate mean + SEM.∗ p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001 (One-Way ANOVA and Mann-Whitney U test).