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. 2019 Oct 30;3(6):e308. doi: 10.1097/HS9.0000000000000308

Figure 6.

Figure 6

Nanobody-Fc constructs are effective ADCC inducers in CLL and HC. HC- or CLL-derived NK cells were co-cultured with untreated Daudi cells, or with Daudi cells pre-treated with VUN401, a CXCR4-Nb-Fc construct (1ng/ml) in indicated E:T ratio's (A) or in a E:T ratio of 1:10 (B–E) for 3 to 4 hours (n = 6). Target cell death, degranulation, granzyme B release, activation and cytokine production were measured by flow cytometry. (A) Percentage specific target cell lysis induced by HC- (open symbols) or CLL-derived (filled symbols) NK cells. (B) Percentage of degranulated (CD107a+) NK cells after co-culture with VUN401-treated Daudi cells. (C) Release of granzyme B of NK cells after co-culture with VUN401-treated Daudi cells. (D) Activation of NK cells after co-culture with VUN401-treated Daudi cells. (E) Percentage of IFNγ producing NK cells after co-culture with VUN401-treated Daudi cells. (F) Percentage specific lysis of CLL cells after VUN401 treatment and co-culture with autologous NK cells (n = 6). Bars indicate mean + SEM. ∗∗p < 0.01, ∗∗∗∗p < 0.0001 (One-Way ANOVA).