Figure 1.

Novel subclusters identified in the bone marrow microenvironment by single-cell RNA sequencing.

Schematic representation of the bone marrow niche showing the main stroma populations: osteoblasts, vascular cells and mesenchymal stromal cells. RNA sequencing of bone marrow niche cells at single-cell resolution leads to the identification of: (1) specific subpopulations within the three main populations showing definite transcriptional skewing and trajectories of maturation; (2) a cycling-cell cluster, which includes cells of all three niche populations and is significantly expandend upon myeloablation.