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Superoxide scavenging enzyme in the buffy coat. DNA methylation of SOD2 promoter was determined by (a) quantifying 5mC levels using the methylated DNA immunoprecipitation technique, with IgG (^) included as an antibody control, and (b) by methylation-specific PCR. (c) Gene transcripts of SOD2 were quantified by qPCR using β-ACTIN a housekeeping gene. *P < 0.05 versus CONT and #P < 0.05 versus PDR.
