a, Brightfield images of cells on each respective pattern strongly staining purple, as an osteogenic marker (left column), or red, as an adipogenic marker (right column). Scale bar: 25 μm. b, Scatter plots for staining intensity of individual cells after color deconvolution of the red and purple channels from 3 substrates (ncells = 759–817). c, Percentage of cells staining positive for only adipogenic or osteogenic markers (mean ± s.e.m.; *** p<0.001; * p<0.05; one-way ANOVA with Tukey HSD).