Table 1.
Bacterium | Primer sequence (5′-3′) | bp | Ann. (°C) |
---|---|---|---|
Bifidobacterium spp. | CTCCTGGAAACGGGTGG | 550 | 56 |
GGTGTTCTTCCCGATATCTACA | |||
B. fragilis group | ATAGCCTTTCGAAAGRAAGAT | 495 | 50 |
CCAGTATCAACTGCAATTTTA | |||
Lactobacillus spp. | AGCAGTAGGGAATCTTCCA | 341 | 55 |
CACCGCTACACATGGAG | |||
A. muciniphila | CAGCACGTGAAGGTGGGGAC | 327 | 60 |
CCTTGCGGTTGGCTTCAGAT | |||
F. prausnitzii | GATGGCCTCGCGTCCGATTAG | 199 | 60 |
CCGAAGACCTTCTTCCTCC | |||
Enterobacteriaceae | CATTGACGTTACCCGCAGAAGAAGC | 195 | 63 |
CTCTACGAGACTCAAGCTTGC |
Ann: annealing temperature.