Table 1.

Experimental values for point spread functions. Note that the magnification and resolving power slightly changes for the multi-immersion lenses used for ctASLM for refractive indices (η) between 1.333 and 1.56, respectively. All numbers presented are the mean ± the standard deviation of the Full-Width Half-Maximum, and the sample size is 5 for each measurement. --- not measured.

Optical Performance
Microscope	NA	Field of View (μm2)	Lateral Resolution (μm)	Axial Resolution (μm)	Field of View (μm2)	Lateral Resolution (μm)	Axial Resolution (μm)
		(η = 1.333)			(η = 1.56)
ctASLMI	0.4	870 × 870	0.95 ± 0.030 (raw, n= 10)	0.95 ± 0.115 (raw, n= 10)	737×737	0.83 ± 0.035 (raw, n= 10)	0.94 ± 0.075 (raw, n= 10)
		870 × 870	0.77 ± 0.033 (deconv., n= 10)	0.75 ± 0.087 (deconv., n= 10)	737×737	0.62 ± 0.032 (deconv., n= 10)	0.72 ± 0.064 (deconv., n= 10)
ctASLM2	0.7	327 × 327	0.48 ± 0.012 (raw, n= 10)	0.48 ± 0.029 (raw, n= 10)	310×310	0.45 ± 0.016 (raw, n= 6)	0.38 ± 0.032 (raw, n= 6)
		327 × 327	0.36 ± 0.012 (deconv., n= 10)	0.37 ± 0.018 (deconv., n= 10)	310×310	0.31 ± 0.018 (deconv., n= 6)	0.26 ± 0.018 (deconv., n= 6)
LSFM1	0.4	870 × 870	1.04 ± 0.04 (raw, n= 5)	6.7 ± 0.4 (raw, n=5)	737×737	---	---
LSFM2	0.7	327 × 327	0.5 ± 0.02 (raw, n= 5)	2.66 ± 0.04 (raw, n= 5)	310×310	---	---
Airy Scan	0.8	---	---	---	---	0.32 ± 0.03 (deconv., n= 5)	3.1 ± 0.4 (deconv., n= 5)