Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 16;12:11057–11068. doi: 10.2147/OTT.S228199

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Cheng et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

NCK1-AS1 expression and effects of NCK1-AS1 silence on proliferation in parental and DDP-resistant osteosarcoma cells. (A) mRNA level of NCK1-AS1 in normal and osteosarcoma cells. (B) mRNA expression of NCK1-AS1 after transfection of with shRNA- NCK1-AS1 vectors in MG63 and MG63-cis cells. (C) Cell viability of shRNA- NCK1-AS1 transfected MG63 and MG63-cis cells was measured by CCK-8 assay. (D) Colony formation assay was carried out to evaluate the capacity of cell proliferation of parental and DDP-resistant MG63cells transfected with shRNA- NCK1-AS1. (E and F) Levels of CDK2, cyclinE1and p21 were detected by Western blot analysis. The data are shown as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Untreated cells; ^##P < 0.01, ^###P < 0.001 vs. Control shRNA group.