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. 2019 Dec 16;12:11057–11068. doi: 10.2147/OTT.S228199

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© 2019 Cheng et al.

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Effects of NCK1-AS1 silence on the DDP sensitivity of MG63 and MG63-cis cells. (A) Cell viability of MG63 and MG63-cis cells transfected with shRNA- NCK1-AS1 was detected by CCK-8 assay in the present of different dose of DDP. (B) Flow cytometric analysis was performed to estimate MG63 and MG63-cis cell apoptosis after transfection with shRNA- NCK1-AS1. Levels of Bax, Bcl-2 and cleaved caspase 3 in MG63 (C) and MG63-cis (D) cells were measured by Western blot analysis. The data are shown as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Untreated cells; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. Control shRNA group.