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. 2019 Dec 16;12:11057–11068. doi: 10.2147/OTT.S228199

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© 2019 Cheng et al.

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NCK1-AS1 directly targets miRNA-137. (A) The binding sequence between NCK1-AS1 and miRNA-137 were predicted putatively. (B) The luciferase activity of wild-type or mutant NCK1-AS1 reporter was detected in MG63 cells transfected with shRNA- NCK1-AS1. (C) Immunoprecipitation (RIP) assay was performed to confirm the relationship between NCK1-AS1 and miRNA-137. The data are shown as the means ± SD. **P < 0.01, ***P < 0.001 vs. Untreated cells.