Table 2. Gene mutation results obtained by cobas and NGS methods in 50 consecutive NSCLC patients referred for T790M resistance mutation analysis due to relapse after TKI treatment.
| PATIENT | EGFR analysis by cobasa | EGFR analysis by NGSb | Other Gene Alterations detected by NGSc |
|---|---|---|---|
| 1 | Ex19Del (12.82) | Ex19Del (p.L747_S752del) [9.62%] | BRAF p.V600K [0.6%] |
| 2 | p.L858R (6.00) | Wild Type | KRAS p.G12V [0.5%] |
| 3 | p.S768l (5.49), p.G719X (2.54) | p.G719S [5.23%] | NONE |
| 4 | p.L858R (6.97) | p.L858R[6.72%] | NONE |
| 5 | p.L861Q (5.21) | p.L861Q [0.38%] | NONE |
| 6 | Ex19Del (19.01) | Ex19Del (p.Glu746_Ala750del) [27.71%] | TP53 p.Y220C [0.9%] |
| 7 | Ex19Del (17.49) | Ex19Del p.Glu746_Ala750del [23.44%] | NONE |
| 8 | Ex19Del (5.99) | Ex19Del p.E746_A750del [9.12%] | TP53 p.R175H [1.1%] |
| 9 | Ex19Del (5.99) | Wild Type | NONE |
| 10 | Ex19Del (10.12) | Ex19Del (p.E746_A750del) [20.57%], p.T790M [0.55%] | NONE |
| 11 | EX19Del (10.96), p.T790M (15.28) | Ex19Del (p.L747_A750delinsP) (24.42%), p.T790M (12.78%) | NONE |
| 12 | EX19Del (6.00), p.T790M (8.33) | Ex19Del(p.L747_T751del) (0.82%), p.T790M (1.24%) | NONE |
| 13 | p.L858R (13.02), p.T790M (16.84) | p.L858R (2.44%), p.T790M (4.55%) | NONE |
| 14 | EX19Del (12.04), p.T790M (17.02) | Ex19Del (p.E746_A750del) (11.55%), p.T790M (10.66%) | NONE |
| 15 | EX19Del (14.56), p.T790M (12.73) | p.L858R (4.45%), p.T790M (3.12%) | NONE |
| 16 | EX19Del, p.T790M (11.2) | Ex19Del (p.L747_T751>Q) (11.52%), p.T790M (2.45%) | NONE |
| 17 | EX19Del (11.36), p.T790M (16.30) | Ex19Del (p.E746_A750del) (2.98%), p.T790M (3.21%) | NONE |
| 18 | EGFR p.T790M (5.66) | Ex19Del (p.E746_A750del) (1.04%), p.T790M (1.56%) | NONE |
| 19 | Wild Type | p.L858R [9.63%], p.T790M [1.24%] | NONE |
| 20 | Wild Type | Wild Type | BRAF p.V600E [0.8%] |
| 21 | Wild Type | Wild Type | NONE |
| 22 | Wild Type | Wild Type | NONE |
| 23 | Wild Type | Wild Type | TP53 p.C176S [1.9%] |
| 24 | Wild Type | Wild Type | NONE |
| 25 | Wild Type | Wild Type | MET amplification [1.3] |
| 26 | Wild Type | Wild Type | NONE |
| 27 | Wild Type | Wild Type | KRAS p.G12D [0.7%] |
| 28 | Wild Type | Wild Type | NONE |
| 29 | Wild Type | Ex19Del (p.E746_A750del) [9.24%], p.T790M [1.65%] | NONE |
| 30 | Wild Type | Wild Type | NONE |
| 31 | Wild Type | Wild Type | NONE |
| 32 | Wild Type | Wild Type | NONE |
| 33 | Wild Type | Wild Type | NONE |
| 34 | Wild Type | Wild Type | MET AMPLIFICATION [1.64] |
| 35 | Wild Type | Wild Type | NONE |
| 36 | Wild Type | Wild Type | NONE |
| 37 | Wild Type | Wild Type | NRAS p.G13S [0.82%] |
| 38 | Wild Type | Ex19Del (p.E746_S752delinsV) [0.72%] | TP53 p.G245D [0.83%], MET p.Y1248H [0.94%], KRAS p.G12C [0.63%], PIK3CA p.E542K [1.02%] |
| 39 | Wild Type | Wild Type | NONE |
| 40 | Wild Type | Wild Type | TP53 p.R249fs [3.82%] |
| 41 | Wild Type | Wild Type | NONE |
| 42 | Wild Type | Wild Type | NONE |
| 43 | Wild Type | Wild Type | TP53 p.R282W [2.54%] |
| 44 | Wild Type | Wild Type | NONE |
| 45 | Wild Type | p.G719A [4.12%] | KRAS p.G12V [2.33%], BRAF p.V600E [1.64%], TP53 p.R175H [1.23%] |
| 46 | Wild Type | Ex19Del (p.E746_S750del) [0.47%] | MAP2K1 p.E203K [0.82%] |
| 47 | Wild Type | Wild Type | NONE |
| 48 | Wild Type | Wild Type | NONE |
| 49 | Wild Type | p.G719A [0.93%] | PIK3CA p.E542G [0.44%], TP53 p.R175H [0.85%], KRAS p.G12C [0.66%] |
| 50 | Wild Type | Wild Type | NONE |
a. The semi-quantitative index of the cobas test is provided in brackets.
b. Allele frequency of EGFR gene detected by the NGS method is given in brackets.
c. Allele frequency of each gene alteration and/or the CNV ratio of each amplification detected is given in brackets.