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. 2019 Dec 20;14(12):e0226865. doi: 10.1371/journal.pone.0226865

Fig 7. Schematic diagram displayed the four novel molecular markers.

Fig 7

The indel makers in the intergenic spacers, including GSPC-F/R, GSPR-F/R, and SCPB-F/R, and ycf1 SNP were verified in Siraitia species with five individuals. (A) Sequencing results showed that PCR amplification by GSPC-F/R between the ndhC and trnV-UAC in S. grosvenorii and S. siamensis were 790 bp and 808 bp, respectively. The difference can also be found by GSPR-F/R (B), and SCPB-F/R (C) between the two Siraitia species. (D) GSPY-F/R marker for ycf1 SNP sites were validated in each case, which were effective to discriminate the two Siraitia species.