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. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570

Fig 4. Small molecules evaluation with the novel reporters.

Fig 4

293T cells were transfected with the indicated reporter plasmid and treated with the listed compounds (10 μM). (A) TOP-NLuc; (B) GLI-FLuc, (C) CBF-Gluc. White bars: uninduced conditions (unind.), grey bars: induction with conditioned medium (A: CM, contains Wnt3a) or by co-transfection (B: phGli1, C: phICN1). CHIR = CHIR99021, LGK = LGK-974, LY = LY2090314, Niclo = niclosamide, Soni = sonidegib, Vismo = vismodegib. (**P≤0.01, ***P≤0.001, ****P≤0.0001, n.s. = not significant, t-test, n = 4) Normalised RLUs were multiplied with 100 (TOP-NLuc, GLI-FLuc and CBF-GLuc).