Figure 5. Viability of assay of C6 cells showing live cells stained with calcein acetoxymethyl ester (green) and dead cells stained with ethidium homodimer-1 (red). (A) In microfluidic device, the two regions of analysis are highlighted: blue, the region I (bifurcation of entrance of fluid on the chip) and, in red, the region II (central cavity of chip); (B and C) Images of microscopy of clear field, showing the cell cultivation in region I and II, respectively. (D and E) Images of fluorescence of live C6 cells (green) before magnetic hyperthermia therapy. (F and G) Images of live C6 cells of fluorescence images (green) and dead cells (red), after 10 minutes of magnetic hyperthermia on the chip. (H and J) Fluorescence images of dead C6 cells (red) in regions I and II of the analysis, after 30 minutes of magnetic hyperthermia therapy. All images presented are composed by overlap of images from analysis of each staining (Calcein acetoxymethyl ester and ethidium homodimer-1) and posterior subtraction of background.