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. Author manuscript; available in PMC: 2019 Dec 20.
Published in final edited form as: Methods Mol Biol. 2013;978:73–92. doi: 10.1007/978-1-62703-293-3_6

Table 2.

DNA display methods. Only the microbead display has been used to evolve enzymes.

Method Principle of attachment DNA - point of attachment Protein fusion partner
Microbead display [23,25,24] Non-covalent binding of DNA to streptavidin microbead and of HA-tagged protein via anti-HA antibody to same bead, IVC is needed Biotinylated HA-tag
STABLE
[62,26]		 Non-covalent attachment of protein to DNA,
IVC is needed		 Biotinylated Streptavidin
CIS-display
[17]		 Non-covalent attachment of protein to DNA
 RepA gene DNA replication initiator (RepA)
Covalent DNA display [63,64] Covalent attachment of enzyme to suicide inhibitor that is linked to DNA, IVC is needed Modified with
5-fluoro-deoxycytidine		 HaeIII methyltransferase
Covalent antibody display [18] Covalent attachment of enzyme to DNA
 P2A gene Endonuclease P2A
SNAP display [65,66] Covalent attachment of enzyme to suicide inhibitor that is linked to DNA, IVC is needed Modified with benzyl guanine SNAP-tag