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. Author manuscript; available in PMC: 2020 Nov 15.
Published in final edited form as: Exp Cell Res. 2019 Sep 19;384(2):111625. doi: 10.1016/j.yexcr.2019.111625

Figure 6: SpMyo1 IQ2 motif recruits calmodulin-related light chain Cam2 in S. pombe.

Figure 6:

(A, B) Colocalization analysis of mGFP-tagged human-yeast myosin-I chimeras (green) and mCherry-tagged calmodulin-like light chain Cam2 (red) in myo1Δ cells. mGFP alone, mGFP-tagged SpMyo1 and chimeras were expressed from plasmids under control of 3xPnmt1 promoter for 12-18 hours in the absence of thiamine.

(A) Single confocal sections through the middle of the cells. The blue Sp IQ+/− labels indicate the presence or the absence of the SpMyo1 IQ2 motif. Scale bars, 1 μm.

(B) Montages of individual patches at 2-second intervals. Scale bars, 0.5 μm. Numbers representing percent colocalization (mean ± SD) of Cam2 with SpMyo1 or SpM-HsT-1 in dynamic actin patches are shown above montages. N=41-63 patches in 3-6 cells.

Yellow arrowheads indicate colocalization of Cam2 with SpMyo1 and SpM-HsT-1 in actin patches. White arrows indicate colocalization of Cam2 with HsM-SpT-2 and HsM-SpT-3 in cortical aggregates. Red arrowheads indicate Cam2-only puncta that form in the absence of SpMyo1 IQ2 motif.