Table 1.
Summary of mean frequencies of tubulin incorporation spots observed by TIRF for various growth and exchange conditions after 15 min of tubulin incorporation. Shown is also the sd factor characterizing the width of the lognormal distribution, as well as the total length of microtubules analyzed and the total number of incorporation spots.
| Tubulin concentration (elongation) [μM] |
Tubulin concentration (incorporation) [μM] |
Total length analyzed (μm) |
Number of incorporation spots | Incorporation frequency (mean */ sd factor) [μm-1] |
|---|---|---|---|---|
| 20 | 7 | 675 | 15 | 0.03 */ 2.4 |
| 20 | 14 | 1558 | 34 | 0.04 */ 2.9 |
| 20 | 20 | 497 | 44 | 0.12 */ 2.2 |
| 20 | 26 | 1463 | 94 | 0.10 */ 2.6 |
| 14 | 20 | 149 | 8 | 0.08 */ 1.8 |
| 20 | 20 | 497 | 44 | 0.12 */ 2.2 |
| 26 | 20 | 377 | 65 | 0.23 */ 2.0 |
| 20 (Xenopus egg extract) | 14 | 3278 | 210 | 0.09 */ 2.8 |