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. 2019 Oct 7;31(12):3057–3072. doi: 10.1105/tpc.19.00263

Figure 4.

Figure 4.

The Chloroplastic Ribosome Marker Protein and the psbA mRNA Localize to the T-Zone after Cell Division in the Dark Phase of the Diel Cycle.

(A) Epifluorescence microscopy images revealed in the premitotic cells at ZT20 (n = 15) that the psbA mRNA (green) was higher in the basal region than the lobes while the chloroplastic ribosomal protein S-21 (magenta) was distributed throughout the chloroplast. In most postmitotic ZT20 cells (n = 103) and postmitotic ZT22 cells (n = 72), the strongest signals from the psbA mRNA and the chloroplastic ribosomal protein S-21 colocalized to the T-zone (arrows). Bar = 5.0 µm.

(B) The AtpB subunit of the chloroplastic ATP synthase (magenta) was not localized with the psbA mRNA in premitotic ZT20 cells (n = 9), in postmitotic ZT20 cells (n = 39), or in ZT22 cells (n = 103). Bar = 5.0 µm.

(C) and (D) The patterns in (A) and (B) were confirmed by plots of the average signal intensities versus the chloroplast axis. Cell numbers were, for the psbA mRNA and S-21, ZT20 premitotic (n = 15), ZT20 postmitotic (n = 54), and ZT22 (n = 72; [C]), and for the psbA mRNA and AtpB, ZT20 premitotic (n = 9), ZT20 postmitotic (n = 54), and ZT22 (n = 112; [D]).