. 2019 Oct 1;31(12):3092–3112. doi: 10.1105/tpc.19.00272

Table 3.

XAT Acyl-Donor Substrate Preference

Lipid Substrate	Relative Activity (%)
Triglycerides	100 ± 2.9
1,2-Diglycerides	31 ± 18
Lysophosphotidylcholine	13.6 ± 1.0^a
Free fatty acids	9.3 ± 1.8
1,3-Diglycerides	3.5 ± 0.2
Monoglycerides	1.8 ± 0.7^a
Phosphotidylcholine	ND

In vitro enzyme assays were performed with 20 μg of recombinant XAT and 5 mM of acyl-donor substrate. Assays were performed at 37°C and pH 8.0 for 4 h in a final assay volume of 200 μL. Data show activity ±se relative to trilinolein (Triglyceride) for n = 3 biological replicates from one representative experiment. Experiment was performed 2 times, with similar results. ND, not detected. Statistically significant difference (P < 0.05).