Figure 4.

Accumulation of Ci Acquisition Proteins in FtsH1 Mutants under Ci Replete/Deplete Conditions.

Protein complexes isolated from membrane fractions of FtsH1down and FtsH1over mutants and their respective wild-type controls (WT) grown under Ci-replete/-deplete conditions (+Ci/–Ci) were analyzed by 2D CN/SDS-PAGE. The 2D gel was stained with Sypro Orange. Each loaded sample contained 5 µg of chlorophyll a. Designation of complexes: RCC(2) and RCC(1), dimeric and monomeric PSII core complexes; ATP synt., ATP synthase; spots indicated by white arrows and numbers: 1, SbtA; 2, CmpA; 3, CupA; 4, NdhH; 5, NdhI; 6, NdhJ; 7, NdhF3; 8, NdhD3; 9, RbcL. Proteins were identified by MS (Supplemental Table) and according to Linhartová et al. (2014) and Zhang et al. (2007). Quantification of several protein spots is shown in Supplemental Figure 3.