Figure 4.

Reactions Catalyzed by CYP706A3 on Floral TPS Products.

Wavy arrows indicate volatile compounds. Black arrows indicate primary and NADPH-dependent activities of CYP706A3 on TPS11 [(+)-α-barbatene and (+)-thujopsene], TPS21, and TPS24 products confirmed in vitro. Primary products 1, 2, 3 and 4 (in black and gray) were validated by NMR spectroscopy and represent the major CYP706A3 products emitted from Arabidopsis flowers. Gray arrows indicate secondary NADPH-dependent activities of CYP706A3 on primary products derived from (+)-α-barbatene and (+)-thujopsene obtained in vitro. Raw formulae of secondary products in gray were deduced from LC-MS spectra. Dashed-dotted gray arrows indicate CYP706A3 putative activities deduced from flower metabolic profiling. Unidentified compounds are indicated by question marks.