Figure 7.

Soluble Products of CYP706A3-Dependent Sesquiterpene Oxygenation Detected in Flower Tissues.

Sesquiterpene oxides from wild-type and CYP706A3 mutant plants were quantified in flower methanol extracts. LC-MS/MS using multiple reaction monitoring of specific MS/MS transitions was targeted to identify sesquiterpene oxides resulting from single or multiple oxidations. For each compound differentially detected in mutant lines, the specific MS/MS transitions used are shown, as well as the expected raw formula. Data are given as means ± se of three biological replicates (pooled inflorescences from individual plants) and expressed relative to Col-0 set at 100%. Statistically significant differences relative to Col-0 are indicated (two-tailed Student’s t test: *P < 0.05, **P< 0.01, ***P < 0.001). Statistics can be found in Supplemental Data Set 2. Representative chromatograms and identification of numbered sesquiterpene oxides are shown in Supplemental Figure 14. FS, flower soluble compounds.