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. 2019 Dec 17;10(66):7031–7042. doi: 10.18632/oncotarget.27345

Figure 1. NF-κB down-regulates Gas6 expression in myeloid cells.

Figure 1

(A) Expression of Gas6 by western blot (normalized to GAPDH) in lungs from WT mice treated with intratracheal clodronate (Clod) or empty (PBS) liposomes on day 0 and day 7 after urethane injection. Lungs were harvested on day 14. (B) mRNA expression of Gas6 in bone marrow-derived macrophages from WT mice that were untreated (U/T) or treated with LPS (L, 100 ng/ml) or/and Bay-117082 (B, 10 uM) for 4 hours. *p<0.05 compared to U/T cells. (C) mRNA expression of Gas6 in lung homogenates and (D) lung macrophages from WT or IKKβΔMye mice at Day 7 after single injection of urethane (n=3 per group). (E) Gas6 mRNA expression in bone marrow-derived macrophages from WT and IKKβΔMye mice after 48-hour incubation in DMEM media (+ 10% FBS) supplemented with 30% conditioned medium from Lewis Lung Carcinoma cells, * p<0.05 compared to WT. Data correspond to the mean ± SEM, n=5 mice/group.