Steps	Condition of the first primer			Condition of the second primer
Initial denaturation	95°C	1 M	1 C	95°C	90 Ss	1 C
Denaturation	95°C	30 Ss	30 Cs	95°C	45 Ss	35 Cs
Annealing	58°C	30 Ss		56°C	45 Ss
Extension	72°C	30 Ss		72°C	60 Ss
Final extension	72°C	5 Ms	1 C	72°C	7 Ms	1 C

M: Minute, S: Second, C: Cycle, °C: Celsius