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. Author manuscript; available in PMC: 2019 Dec 21.
Published in final edited form as: Arch Toxicol. 2017 Dec 8;92(3):1049–1064. doi: 10.1007/s00204-017-2142-3

Fig. 12 – Effects of Cd on mesangial cells transfected with CAP1 constructs.

Fig. 12 –

A) Mesangial cells were transfected with an empty vector or vectors containing either His- tagged wt CAP1 or CAP1 C29A mutant. An anti-His Western blot is shown of extracts from cells treated with increasing amounts of Cd. Monomeric CAP1 expression is evident in all wt and C29A transfectants whereas the upper dimeric band(s) appears only in the wt-transfected cells. A blot of -actin is included as a loading control. B)-E) rhodamine-conjugated phalloidin (red), anti-His (green), and DAPI (blue) staining of transfected cells. B) Cells transfected with wt CAP1. The arrow shows a shrunken His- positive cell. C) Cells transfected with the C29A mutant construct. D) Cells transfected with wt CAP1 and exposed to 10 μM CdCl2 in serum-free conditions for 6 h. E) Cells transfected with C29A mutant CAP1 and exposed to 10 μM CdCl2 in serum-free conditions for 6 h.