Table 1 – Effect of CAP1 silencing on RMC viability.

RMC were transfected with either a scrambled-sequence control plasmid (SC) or an siRNA targeting CAP1, as described in Materials and Methods. Cells were then treated with the indicated concentration of CdCl₂ for 6 h in serum-free medium and viability was measured by the MTT assay. The results are mean ± s.d. from quadruplicate wells at each condition, in a single experiment, taking the absorbance of the Cd-free control as 100 % in each data set. In the 3rd and 5th columns, Dunnett’s post hoc test is used to compare the value obtained at each [Cd²⁺] to the value at [Cd²⁺] = 0. The last column shows the results of the Tukey post hoc test on the full data set, comparing scrambled control to silenced cells at the same [Cd²⁺]. NS - not significant (p > 0.05).

[Cd2+] (μM)	SC (%)	p vs [Cd] = 0 (SC) (Dunnett)	siRNA (%)	p vs [Cd] = 0 (si) (Dunnett)	p, SC vs siRNA (Tukey)
		(Dunnett)		(Dunnett)	(Tukey)
0	99.9 ± 7.2	-	100 ± 2.8	-	-
10	109 ± 2.5	NS	107 ± 6.0	NS	NS
20	50.6 ± 6.8	< 0.01	70.7 ± 1.8	< 0.01	< 0.001
40	31.3 ± 3.2	< 0.01	51.9 ± 4.3	< 0.01	< 0.001