Fig. 1.

Diagram presenting polyethylene glycol (PEG) mediated ex-vivo cell fusion procedure to create murine Dystrophin Expressing Chimeric Cell (DEC) and systemic-intraosseous transplantation of murine DEC to themdxmice. a Ex vivo created DEC derived from the wild type snj myoblasts (MB^wt) and dystrophin-deficient mdx (MB^mdx) mice. b Representative flow cytometry plots presenting fusion of the PKH26 labeled MB^wt and PKH67 labeled MB^mdx parent myoblasts assessed by FACS. The overlapping fluorescence of PKH26/PKH67 confirms chimeric state for MB^wt/MB^mdx DEC cell line (Siemionow M. et al. Stem Cell Rev. 2018 Apr;14(2):189–199 [32], http://creativecommons.org/licenses/by/4.0/). c Ex-vivo created DEC derived from the wild type snj myoblasts (MB^wt) and mesenchymal stem cells from dystrophin-deficient mdx (MSC^mdx) mice. d Representative flow cytometry plots presenting fusion of the PKH26 labeled MB^wt and PKH67 labeled MSC^mdx parent cells assessed by FACS. The overlapping fluorescence of PKH26/PKH67 confirms chimeric state for MB^wt/MSC^mdx DEC cell line