Table 1.
Nucleotide sequences, amplification sites, GenBank accession numbers, amplimer sizes and PCR reaction conditions for each primer pair are shown
| Gene | Primer | Nucleotide sequence (5′ → 3′) | GenBank ID | Annealing temperature (°C) | Amplimer size (bp) |
|---|---|---|---|---|---|
| preproPACAP | Sense | GAA GAC GAG GCT TAC GAC CA (314–333) | NM_001315503.1 | 58 | 288 |
| Antisense | GTC CGA GTG GCG TTT GGT (584–601) | ||||
| PAC1 | Sense | TATTACTACCTGTCGGTGAAG(912–932) | NM_007407.4 | 52 | 213 |
| Antisense | ATGACTGCTGTCCTGCTC (1107–1124) | ||||
| VPAC1 | Sense | TTT GAG GAT TTC GGG TGC (974–991) | NM_011703.4 | 53 | 266 |
| Antisense | TGG GCC TTA AAG TTG TCG (1222–1239) | ||||
| VPAC2 | Sense | CTC CTG GTA GCC ATC CTT (805–822) | NM_009511.2 | 53 | 149 |
| Antisense | ATG CTG TGG TCG TTT GTG (936–953) | ||||
| PKA (Prkaca) | Sense | GCAAAGGCTACAACAAGGC (847–865) | NM_008854 | 53 | 280 |
| Antisense | ATGGCAATCCAGTCAATCG (1109–1126) | ||||
| CREB (Creb1) | Sense | AGATTGCCACATTAGCCC (95–112) | NM_031017.1 | 52 | 441 |
| Antisense | GCTGTATTGCTCCTCCCT (518–535) | ||||
| Sox9 | Sense | GTA CCC GCA TCT GCA CAA CG (378–397) | NM_011448 | 62 | 521 |
| Antisense | GTG GCA AGT ATT GGT CAA ACT CAT T (874–898) | ||||
| Sox6 | Sense | GGA GTC GGG AGC GTG AAA (483–500) | NM_001277326.1 | 54 | 381 |
| Antisense | GGC GAG CAA GGT CCA TTT (846–863) | ||||
| Sox5 | Sense | GCT CCA TAC AAC TCA TCT AC (504–523) | NM_011444.3 | 53 | 181 |
| Antisense | TGT CTT CTG GCT CAT TCT (667–684) | ||||
| Aggrecan | Sense | CGG GAA GGT TGC TAT GGT G (782–800) | NM_007424.2 | 59 | 359 |
| Antisense | CCT GTC TGG TTG GCG TGT A (1122–1140) | ||||
| Col2a1 | Sense | AAA GAC GGT GAG ACG GGA GC (1900–1919) | NM_001113515 | 63 | 289 |
| Antisense | GAC CAT CAG TAC CAG GAG TGC C (2167–2188) | ||||
| Chst11 | Sense | TGC TAT GTG CCC AAG GTA (822–839) | NM_021439.2 | 55 | 466 |
| Antisense | CGA GGT CGT AGT GGA TGTG (1269–1287) | ||||
| HAS2 | Sense | ACAGGCATCTCACGAACC (1479–1496) | NM_008216.3 | 51 | 415 |
| Antisense | ATC TTG GCG GGA AGT AAA (1876–2893) | ||||
| HAS3 | Sense | TCGGCGATTCGGTGGACT (835–852) | NM_001331048.1 | 59 | 280 |
| Antisense | TGCTGGAGGAGGCTGTTGC (1096–1114) | ||||
| HAPLN1 | Sense | GGC TCA GGA ATC CAC AAA (217–234) | BC066853 | 55 | 284 |
| Antisense | GGA AAG TAA GGG AAC ACC A (482–500) | ||||
| RHAMM | Sense | GAG GGA CTC AGG ACA AAC (374–391) | NM_013552.2 | 49 | 485 |
| Antisense | TTC TTC TAA CTG GGC AAT (8412–858) | ||||
| CD44 | Sense | GGATTCATCCCAACGCTAT (600–618) | NM_009851.2 | 53 | 216 |
| Antisense | ACT CGC CCT TCT TGC TGT (798–815) | ||||
| Col1a1 | Sense | GGG CGA GTG CTG TGC TTT (237–254) | BC050014 | 62 | 388 |
| Antisense | GGG ACC CAT TGG ACC TGA A (606–624) | ||||
| Col10a1 | Sense | TTC TGG GAT GCC GCT TGT C (1602–1620) | NM_009925 | 61 | 263 |
| Antisense | TCG TAG GCG TGC CGT TCT T (1846–1864) | ||||
| Actin (Actb) | Sense | GCCAACCGTGAAAAGATGA (419–437) | NM_007393.5 | 54 | 462 |
| Antisense | CAAGAAGGAAGGCTGGAAAA (861–880) |