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. 2019 Dec 20;9:19579. doi: 10.1038/s41598-019-55447-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Resistant lines present higher parasite-specific IgA titres levels in saliva than susceptible lambs at the initial period of infection. Saliva samples from infected (A,C) or control (B,D) susceptible (S) and resistant (R) Corriedale and resistant Rylington x Corriedale lambs were incubated with larvae (A,B) or adult (C,D) derived-antigen coated plates. Specific antibodies were detected with anti-sheep IgA HRP-conjugated antibody. Asterisks indicate statistically significant differences (p < 0.05) between resistant Corriedale and susceptible Corriedale lines (indicated by *) or between resistant Rylington x Corriedale and susceptible Corriedale lines (indicated by **).