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. 2019 Dec 20;4:23. doi: 10.1038/s41536-019-0085-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Sca-1 immunostaining for stem/progenitor cells at d3 revealed broad presence of non Scx-lin cells throughout the injury site and colocalization with few Scx-lin cells a. Scx-lin cells colocalized with Sca-1 at d14 b and d56 c with decreased Sca-1 expression at the d56 injury site compared to d3 and d14. The presence of non Scx-lin, ScxGFP+, Sca-1 expressing cells at day 56 points towards contributions of non Scx-lin stem/progenitors that are potentially recruited to the injury site and differentiate to a ScxGFP cell phenotype. Scale = 100 μm.