Figure 1.

(a) A flowchart of the contest. Each group (G1-G16) proposed 400 compounds (cmpds) with a prioritized rank from compound library using their own methods. The proposed compounds that were not stocked-out were selected until the number of compounds reached 200 for each group. If there was duplication in the proposed compounds among different groups, these groups attained additional compounds to be assayed. For this reason, there are differences among the number of selected compounds of each group. Finally, the selected compounds were assayed. (b) The screening flow of the compounds in the experimental assay. The filtering criteria are shown in a trapezium. The number of compounds applied to each screening is shown in parenthesis. This flow was conducted twice with NAD⁺ (the first number in parenthesis) and without NAD⁺ (the second number in parenthesis). (c) IC₅₀ hits found based on TSA screening without (w/o) and with (w/) NAD⁺ (see Screening of potential inhibitors in the main text).