Figure 7.

Summarizing various observations. 1) Black arrows show the flux of pregnane intermediates in ΔAPAT_pL1_hc yeast (L1); 2) Hashed arrows indicate reactions possibly sustained by yeast‐borne enzymes; 3) Pregnenolone metabolism in ΔAPAT_pL1_hc yeast and four lines derived thereof that were cotransformed with a plasmid containing an ADR/ADX gene (L1601). The pregnane patterns (%) detected in the medium after 5 d of incubation at 30 °C are shown (n= mean ± SD), for better comparison values of two strains (L1 and L1601A are shown in red as percentages); 4) Red arrows show conversions not observed in ΔAPAT_CYP21A1, only progesterone seems to be a substrate for MmCYP21A1